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Product Name
GAD65 antibody
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Description
GAD65 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 65 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
GAD 65 antibody; GAD2 antibody; GAD65 antibody; Glutamate decarboxylase 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_000818). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110818(GAD2 antibody) at dilution of 1:1000
Immunofluorescent analysis of HeLa cells using Catalog No:110818(GAD2 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
IP Result of anti-GAD2 (IP:Catalog No:110818, 4ug; Detection:Catalog No:110818 1:1000) with mouse brain tissue lysate 3440ug.
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Background
GAD2, also named as GAD65, belongs to the group II decarboxylase family. GAD2 catalyzes the production of GABA. It is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid. GAD2 is expressed in nervous and endocrine systems and are thought to be involved in synaptic transmission and insulin secretion. Autoantibodies against GAD2 may serve as markers for type I diabetes. Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2. The antibody is specific to GAD2.
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References
- Liu H, Li Y, Wang Y. The distinct role of NR2B subunit in the enhancement of visual plasticity in adulthood. Molecular brain. 8:49. 2015.
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