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Product Name
GABPB1 antibody
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Description
GABPB1 Rabbit Polyclonal antibody. Positive WB detected in mouse lung tissue, HeLa cells, HepG2 cells, MCF7 cells, mouse testis tissue. Positive IP detected in HeLa cells. Positive IHC detected in human testis tissue. Observed molecular weight by Western-blot: 36-45 kDa,47-53 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BABPB2 antibody; E4TF1 antibody; E4TF1 47 antibody; E4TF1 53 antibody; E4TF1B antibody; GABP subunit beta 1 antibody; GABP subunit beta 2 antibody; GABPB antibody; GABPB 1 antibody; GABPB 2 antibody; GABPB1 antibody; GABPB2 antibody; NRF2B1 antibody; NRF2B2 antibody; Nuclear respiratory factor 2 antibody; Transcription factor E4TF1 47 antibody; Transcription factor E4TF1 53 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GABPB1 recombinant protein (Accession Number: NM_002041). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:110805(GABPB1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human testis using Catalog No:110805(GABPB1 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-GABPB1 (IP:Catalog No:110805, 4ug; Detection:Catalog No:110805 1:1000) with HeLa cells lysate 520ug.
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Background
GABPB1, also named as E4TF1B, GABPB, GABPB2, E4TF1-47 and E4TF1-53, is a transcription factor capable of interacting with purine rich repeats (GA repeats). It is necessary for the expression of the Adenovirus E4 gene. GABPB1 has 4 isoforms. The MW-of Beta 1 is 53kd and Beta-2 is 47kd(PMID:9525935). This antibody can recognize all the isoforms.
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References
- Wu H, Xiao Y, Zhang S. The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense. Cell reports. 3(5):1663-77. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"