Cart 2

GABARAP antibody

Cat.#: 110770

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    GABARAP antibody
  • Documents
  • Description
    GABARAP Rabbit Polyclonal antibody. Positive IF detected in Starvation treated HepG2 cells. Positive WB detected in human placenta tissue, A549 cells, HeLa cells, human heart tissue, mouse brain tissue, mouse liver tissue, mouse pancreas tissue, mouse skeletal muscle tissue. Observed molecular weight by Western-blot: 14 kDa
  • Tested applications
    ELISA, WB, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    FLC3B antibody; FLJ25768 antibody; GABARAP antibody; MM46 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_007278). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IF: 1:20-1:200

  • Validations

    human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:110770(GABARAP antibody) at dilution of 1:500

    human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:110770(GABARAP antibody) at dilution of 1:500

    Immunofluorescent analysis of (-20oc Ethanol) fixed Starvation treated HepG2 cells using Catalog No:110770(GABARAP Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (-20oc Ethanol) fixed Starvation treated HepG2 cells using Catalog No:110770(GABARAP Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    GABARAP (Gamma-aminobutyric acid type A receptor-associated protein), was initially identified as GABAA receptor binding protein and has shown to be implicated in intracellular trafficking of GABAA receptors. Through interacting with a broad rang of proteins, GABARAP has been demonstrated as a multifunctional protein implicated in intracellular vesicle trafficking, apoptosis, and autophagy. The protein level of lipidated form of GABARAP (GABARAP-II) is upregulated during the autophagic process, which makes it as an autophagic marker. This antibody detected endogenous GABARAP protein in lysates from various tissues. It may cross-react with GABARAPL1 due to the high identity of amino acid sequence.
  • References
    • Fetalvero KM, Yu Y, Goetschkes M. Defective autophagy and mTORC1 signaling in myotubularin null mice. Molecular and cellular biology. 33(1):98-110. 2013.
    • Labonté D, Thies E, Kneussel M. The kinesin KIF21B participates in the cell surface delivery of γ2 subunit-containing GABAA receptors. European journal of cell biology. 93(8-9):338-46. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"