-
Product Name
GAA Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to GAA
-
Tested applications
WB, IF, IP
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
GAA antibody; LYAG antibody; glucosidase alpha, acid antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 350-450 of human GAA (NP_000143.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:200 -
Validations
Western blot - GAA Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GAA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
-
Background
Essential for the degradation of glycogen in lysosomes. Has highest activity on alpha-1,4-linked glycosidic linkages, but can also hydrolyze alpha-1,6-linked glucans.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"