-
Product Name
FZR1 antibody
- Documents
-
Description
FZR1 Rabbit Polyclonal antibody. Positive IP detected in mouse heart tissue. Positive WB detected in NIH/3T3 cells, C6 cells, HeLa cells, HepG2 cells, Jurkat cells, mouse heart tissue, mouse liver tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 55 kDa
-
Tested applications
ELISA, WB, IP, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
CDC20 like protein 1 antibody; CDC20C antibody; CDH1 antibody; Cdh1/Hct1 homolog antibody; Fizzy related protein homolog antibody; FYR antibody; FZR antibody; FZR1 antibody; FZR2 antibody; HCDH antibody; HCDH1 antibody; KIAA1242 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of FZR1 recombinant protein (Accession Number: NM_016263). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:50-1:500
-
Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:110761(FZR1 Antibody) at dilution of 1:600
IP Result of anti-FZR1 (IP:Catalog No:110761, 4ug; Detection:Catalog No:110761 1:500) with mouse heart tissue lysate 3200ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:110761(FZR1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
FZR1 gene, also named as FYR, FZR and KIAA1242, has been previously shown to be mutated in lobular breast carcinomas. Direct evidence of FZR1 mutations triggering tumorigenesis has been investigated. FZR1 is a key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C) which is playing vital role s in cell cycle progression. There are 7 WD-repeat domains in FZR1. Upon phosphorylation and dephosphorylation, it interacts with other components to modulate cell mitosis. This gene is different to E-cadherin (CDH1)which is an epithelial cell adhesion molecule.
-
References
- Perry MC, Dufour CR, Eichner LJ. ERBB2 deficiency alters an E2F-1-dependent adaptive stress response and leads to cardiac dysfunction. Molecular and cellular biology. 34(23):4232-43. 2014.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"