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Product Name
FUT6 Polyclonal Antibody
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Description
Polyclonal antibody to FUT6
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Tested applications
WB, IHC
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Species reactivity
Human
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Alternative names
FUT6 antibody; FCT3A antibody; FT1A antibody; Fuc-TVI antibody; FucT-VI antibody; fucosyltransferase 6 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 170-359 of human FUT6 (NP_000141.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:100 - 1:200 -
Validations
Western blot - FUT6 Polyclonal Antibody
Western blot analysis of extracts of SW480 cells, using FUT6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - FUT6 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver using FUT6 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - FUT6 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using FUT6 antibody at dilution of 1:100 (40x lens).
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Background
Enzyme involved in the biosynthesis of the E-Selectin ligand, sialyl-Lewis X. Catalyzes the transfer of fucose from GDP-beta-fucose to alpha-2,3 sialylated substrates.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"