-
Product Name
FUT3 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to FUT3
-
Tested applications
WB, IF
-
Species reactivity
Human, Rat
-
Alternative names
FUT3 antibody; CD174 antibody; FT3B antibody; FucT-III antibody; LE antibody; Les antibody; galactoside 3-L-fucosyltransferase antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FUT3 (NP_000140.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - FUT3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using FUT3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
-
Background
May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"