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Product Name
FTSJ1 Polyclonal Antibody
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Description
Polyclonal antibody to FTSJ1
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Tested applications
WB
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Species reactivity
Human
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Alternative names
FTSJ1 antibody; CDLIV antibody; JM23 antibody; MRX44 antibody; MRX9 antibody; SPB1 antibody; TRMT7 antibody; FtsJ RNA methyltransferase homolog 1 (E. coli) antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 170-329 of human FTSJ1 (NP_036412.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - FTSJ1 Polyclonal Antibody
Western blot analysis of extracts of HT-1080 cells, using FTSJ1 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
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Background
Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"