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FRS2 antibody

Cat.#: 110777

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Product Information

  • Product Name
    FRS2 antibody
  • Documents
  • Description
    FRS2 Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue. Positive IF detected in MCF-7 cells. Positive WB detected in MCF-7 cells, human brain tissue, NIH/3T3 cells. Observed molecular weight by Western-blot: 68 kDa
  • Tested applications
    ELISA, IHC, WB, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    FGFR signaling adaptor SNT antibody; FGFR substrate 2 antibody; FRS2 antibody; FRS2A antibody; FRS2alpha antibody; SNT antibody; SNT 1 antibody; SNT1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of FRS2 recombinant protein (Accession Number: NM_006654). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:110777(FRS2 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:110777(FRS2 antibody) at dilution of 1:50 (under 10x lens)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with Catalog No:110777(FRS2 Antibody) at dilution of 1:1000

    MCF-7 cells were subjected to SDS PAGE followed by western blot with Catalog No:110777(FRS2 Antibody) at dilution of 1:1000

    Immunofluorescent analysis of MCF-7 cells using Catalog No:110777(FRS2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of MCF-7 cells using Catalog No:110777(FRS2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) has an alternative name as SNT-1, it is an adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. FGF receptor substrates (FRS2 and FRS3) are key adaptor proteins that mediate FGF-FGFR signalling in benign as well as malignant tissue. FRS2 is a 508 amino-acid protein,which is phosphorylated on tyrosine residues. The molecular weight of non-phosphorylated FRS2 is 57-68 kDa, but phosphorylated FRS2 is 80-90 kDa. Phosphorylation of FRS2 is associated with activation of a number of MAP kinases. Allele-specific regulation of FGFR2 mRNA expression with a mildly increased breast cancer risk has been reported.
  • References
    • Wang X, Asmann YW, Erickson-Johnson MR. High-resolution genomic mapping reveals consistent amplification of the fibroblast growth factor receptor substrate 2 gene in well-differentiated and dedifferentiated liposarcoma. Genes, chromosomes & cancer. 50(11):849-58. 2011.
    • Zhang K, Chu K, Wu X. Amplification of FRS2 and activation of FGFR/FRS2 signaling pathway in high-grade liposarcoma. Cancer research. 73(4):1298-307. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"