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Product Name
FRK Polyclonal Antibody
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Description
Polyclonal antibody to FRK
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
FRK antibody; GTK antibody; PTK5 antibody; RAK antibody; tyrosine-protein kinase FRK antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human FRK (NP_002022.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - FRK Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using FRK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - FRK Polyclonal Antibody
Immunofluorescence analysis of A549 cells using FRK antibody . Blue: DAPI for nuclear staining.
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Background
Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"