-
Product Name
FREM1 antibody
- Documents
-
Description
FREM1 Rabbit Polyclonal antibody. Positive WB detected in human plasma tissue, HepG2 cells. Positive IHC detected in human liver cancer tissue, human breast cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 40-43 kDa; 70-80 kDa
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human; other species not tested.
-
Alternative names
C9orf143 antibody; C9orf145 antibody; C9orf154 antibody; FLJ25461 antibody; FREM1 antibody; Protein QBRICK antibody; QBRICK antibody; RP11 439N12.3 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of FREM1 recombinant protein (Accession Number: XM_006716729). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
human plasma tissue were subjected to SDS PAGE followed by western blot with Catalog No:110720(FREM1 Antibody) at dilution of 1:300
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:110720(FREM1 Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:110720(FREM1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:110720(FREM1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:110720(FREM1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
FREM1, C9orf143, C9orf145, C9orf154 and QBRICK, is an extracellular matrix protein that plays a role in epidermal differentiation and is required for epidermal adhesion during embryonic development. This antibody recognizes isoform 1 (244 kDa), isform 2 (80 kDa) and isoform 3 (43 kDa).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"