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Product Name
FOXP3 antibody
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Description
FOXP3 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, A375 cells, HEK-293 cells, mouse brain tissue, rat brain tissue, rat pancreas tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 50-55 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AIID antibody; DIETER antibody; forkhead box P3 antibody; Forkhead box protein P3 antibody; FOXP3 antibody; IPEX antibody; JM2 antibody; PIDX antibody; Scurfin antibody; XPID antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FOXP3 recombinant protein (Accession Number: NM_014009). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:20-1:200
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Validations
WB results of Catalog No:110711(FOXP3 antibody).
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110711(FOXP3 antibody) at dilution of 1:2000
IP Result of anti-FOXP3 (IP:Catalog No:110711, 3ug; Detection:Catalog No:110711 1:1000) with HeLa cells lysate 1840ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:110711(FOXP3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. POXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human FOXP3.
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References
- Hao J, Zhang C, Liang T, Song J, Hou G. rFliC prolongs allograft survival in association with the activation of recipient Tregs in a TLR5-dependent manner. Cellular & molecular immunology. 11(2):206-14. 2014.
- Zhao M, Wang LT, Liang GP. Up-regulation of microRNA-210 induces immune dysfunction via targeting FOXP3 in CD4(+) T cells of psoriasis vulgaris. Clinical immunology (Orlando, Fla.). 150(1):22-30. 2014.
- Yan C, Zhang BB, Hua H. The Dynamics of Treg/Th17 and the Imbalance of Treg/Th17 in Clonorchis sinensis-Infected Mice. PloS one. 10(11):e0143217. 2015.
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