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Product Name
FMR1 antibody
- Documents
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Description
FMR1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in K-562 cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse brain tissue, mouse thymus tissue. Positive IHC detected in human gliomas tissue. Positive IF detected in HepG2 cells, Hela cells. Observed molecular weight by Western-blot: 66-71kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FMR1 antibody; FMRP antibody; fragile X mental retardation 1 antibody; FRAXA antibody; POF antibody; POF1 antibody; Protein FMR 1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FMR1 recombinant protein (Accession Number: BC038998). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:110732(FMR1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:110732(FMR1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-FMR1 (IP:Catalog No:110732, 4ug; Detection:Catalog No:110732 1:1000) with HeLa cells lysate 2800ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:110732(FMR1 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"