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Product Name
FMO3 Polyclonal Antibody
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Description
Polyclonal antibody to FMO3
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
FMO3 antibody; FMOII antibody; TMAU antibody; dJ127D3.1 antibody; flavin containing monooxygenase 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 263-532 of human FMO3 (NP_001002294.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:10 - 1:100 -
Validations
Western blot - FMO3 Polyclonal Antibody
Western blot analysis of extracts of mouse liver, using FMO3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - FMO3 Polyclonal Antibody
Immunofluorescence analysis of A-549 cells using FMO3 antibody . Blue: DAPI for nuclear staining.
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Background
Essential hepatic enzyme that catalyzes the oxygenation of a wide variety of nitrogen- and sulfur-containing compounds including drugs as well as dietary compounds. Plays an important role in the metabolism of trimethylamine (TMA), via the production of trimethylamine N-oxide (TMAO) metabolite. TMA is generated by the action of gut microbiota using dietary precursors such as choline, choline containing compounds, betaine or L-carnitine. By regulating TMAO concentration, FMO3 directly impacts both platelet responsiveness and rate of thrombus formation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"