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Product Name
FLNC Polyclonal Antibody
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Description
Polyclonal antibody to FLNC
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
FLNC antibody; ABP-280 antibody; ABP280A antibody; ABPA antibody; ABPL antibody; CMH26 antibody; FLN2 antibody; MFM5 antibody; MPD4 antibody; RCM5 antibody; filamin-C antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 2160-2340 of human FLNC (NP_001449.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - FLNC Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using FLNC antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Immunofluorescence - FLNC Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using FLNC antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Background
Muscle-specific filamin, which plays a central role in muscle cells, probably by functioning as a large actin-cross-linking protein. May be involved in reorganizing the actin cytoskeleton in response to signaling events, and may also display structural functions at the Z lines in muscle cells. Critical for normal myogenesis and for maintaining the structural integrity of the muscle fibers.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"