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Product Name
FLII Polyclonal Antibody
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Description
Polyclonal antibody to FLII
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
FLII antibody; FLI antibody; FLIL antibody; Fli1 antibody; protein flightless-1 homolog antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human FLII (NP_002009.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - FLII Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using FLII antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5S.
Immunohistochemistry - FLII Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using FLII antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - FLII Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon carcinoma using FLII antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - FLII Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using FLII antibody at dilution of 1:100 (40x lens).
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Background
May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (By similarity). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"