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FLI1 antibody

Cat.#: 110687

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Product Information

  • Product Name
    FLI1 antibody
  • Documents
  • Description
    FLI1 Rabbit Polyclonal antibody. Positive IHC detected in human lymphoma tissue. Positive WB detected in HL-60 cells, human brain tissue, Jurkat cells, mouse thymus tissue. Observed molecular weight by Western-blot: 51 kDa
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    EWSR2 antibody; FLI1 antibody; Proto oncogene Fli 1 antibody; SIC 1 antibody; Transcription factor ERGB antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of FLI1 recombinant protein (Accession Number: NM_002017). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:50-1:500

  • Validations

    HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:110687(FLI1 antibody) at dilution of 1:1000

    HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:110687(FLI1 antibody) at dilution of 1:1000

    Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using Catalog No:110687(FLI1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using Catalog No:110687(FLI1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using Catalog No:110687(FLI1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using Catalog No:110687(FLI1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

  • Background
    Friend leukemia virus integration1 (FLI1) that is also termed EWSR2 encodes a sequence-specific transcriptional activator involved in Ewing’s sarcoma (EWS), a malignant small, round, blue cell tumour. It is a rare disease in which cancer cells are found in the bone or in soft tissue. The most common areas in which it occurs are the pelvis, the femur, the humerus, the ribs and clavicle. And EWS occurs most frequently in teenagers and young adults. FLI1 protein may act as an aberrant transcription factor, but exact mechanisms of oncogenesis remains unknown.
  • References
    • Zhang J, Guo H, Zhang H. Putative tumor suppressor miR-145 inhibits colon cancer cell growth by targeting oncogene Friend leukemia virus integration 1 gene. Cancer. 117(1):86-95. 2011.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"