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Product Name
Flag tag antibody
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Description
Flag tag Rabbit Polyclonal antibody. Positive IF detected in Transfected HEK-293 cells. Positive WB detected in recombinant protein.
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Tested applications
ELISA, WB, IF
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Species reactivity
Tag
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Flag tag recombinant protein. Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:4000
IF: 1:10-1:100
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Validations
Western blot of Flagged fusion protein with anti-Flag (Catalog No:117313) at various dilutions.
Immunofluorescent analysis of Transfected HEK-293 cells using Catalog No:117313(Flag tag Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (3x)DYKDDDDK peptide and detects FLAG-tagged proteins.
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References
- Fang G, Zhang D, Yin H, Zheng L, Bi X, Yuan L. Centlein mediates an interaction between C-Nap1 and Cep68 to maintain centrosome cohesion. Journal of cell science. 127(Pt 8):1631-9. 2014.
- Zatyka M, Da Silva Xavier G, Bellomo EA. Sarco(endo)plasmic reticulum ATPase is a molecular partner of Wolfram syndrome 1 protein, which negatively regulates its expression. Human molecular genetics. 24(3):814-27. 2015.
- Zhou Y, Li S, Mäyränpää MI. OSBP-related protein 11 (ORP11) dimerizes with ORP9 and localizes at the Golgi-late endosome interface. Experimental cell research. 316(19):3304-16. 2010.
- Ning J, Zhang B, Wang N, Zhou Y, Xiong L. Increased leaf angle1, a Raf-like MAPKKK that interacts with a nuclear protein family, regulates mechanical tissue formation in the Lamina joint of rice. The Plant cell. 23(12):4334-47. 2011.
- Cai R, Xue W, Liu S, Petersen RB, Huang K, Zheng L. Overexpression of glyceraldehyde 3-phosphate dehydrogenase prevents neurovascular degeneration after retinal injury. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 29(7):2749-58. 2015.
- Liao XH, Wang N, Zhao DW. STAT3 Protein Regulates Vascular Smooth Muscle Cell Phenotypic Switch by Interaction with Myocardin. The Journal of biological chemistry. 290(32):19641-52. 2015.
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