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Product Name
Flag tag antibody
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Description
Flag tag Mouse Monoclonal antibody. Positive IF detected in Transfected HEK-293 cells. Positive WB detected in Recombinant protein, Transfected HEK-293 cells. Positive IP detected in Recombinant protein.
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human; other species not tested.
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Alternative names
DDK tag antibody; DYKDDDDK antibody; FLAG antibody; Flag tag antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of Peptide. Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:5000-1:50000
IP: N/AIF: 1:20-1:200
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Validations
IP Result of anti-Flag tag (IP:Catalog No:117312, 3ug; Detection:Catalog No:117313 1:2000) with Recombinant protein protein lysate 1280ug.
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:117312(Flag tag Antibody) at dilution of 1:1000
Recombinant protein were Recombinant protein were subjected to SDS PAGE followed by western blot with Catalog No:117312 (Flag tag Antibody) at various dilutions.
Immunofluorescent analysis of Transfected HEK-293 cells using Catalog No:117312(Flag tag Antibody) at dilution of 1:50 and Alexa Fluor 594-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
Flag tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDDDDK. The structure of Flag tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, the Flag tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xFlag tag (DYKDDDDK) and can recognize protein containing one or more Flag tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with Flag tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 1E7B4, which is different from any other vendors.
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References
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- Li C, Wang L, Zhang J. CERKL interacts with mitochondrial TRX2 and protects retinal cells from oxidative stress-induced apoptosis. Biochimica et biophysica acta. 1842(7):1121-9. 2014.
- Liao XH, Wang N, Liu LY. MRTF-A and STAT3 synergistically promote breast cancer cell migration. Cellular signalling. 26(11):2370-80. 2014.
- Liao XH, Wang N, Zhao DW. NF-κB (p65) negatively regulates myocardin-induced cardiomyocyte hypertrophy through multiple mechanisms. Cellular signalling. 26(12):2738-48. 2014.
- Zhao L, Zhu J, Zhou H. Identification of cellular microRNA-136 as a dual regulator of RIG-I-mediated innate immunity that antagonizes H5N1 IAV replication in A549 cells. Scientific reports. 5:14991. 2015.
- Su C, Zhang J, Zheng C. Herpes simplex virus 1 UL41 protein abrogates the antiviral activity of hZAP by degrading its mRNA. Virology journal. 12:203. 2015.
- Chen MC, Huang HH, Lai CY. Novel histone deacetylase inhibitor MPT0G009 induces cell apoptosis and synergistic anticancer activity with tumor necrosis factor-related apoptosis-inducing ligand against human hepatocellular carcinoma. Oncotarget. 7(1):402-17. 2016.
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