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Product Name
Human Fibronectin (His Tag) recombinant protein
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Description
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
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Protein name
Fibronectin
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Protein short names
CIG; DKFZP686H0342; FN-1; FIBRONECTIN 1; MSF; GFND; E330027I09; DKFZP686F10164; GFND2; DKFZP686I1370; ED-B; DKFZP686O13149; FN1; FN; MGC117493; LETS; FNZ; FINC
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Uniprot ID
P02751
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Gene Name
FN1; FN
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the Fragment 2 (Ser 607-Pro 1265) of humanFibronectin (CAD91166) was expressed with a C-terminal polyhistidine tag.
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Protein Species
Human
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Molecular weight
The secreted recombinant human Fibronectin 1 fragment2 (FN1.2) consists of 670 amino acids and has a calculated molecular mass of 73.2 kDa. As a result of glycosylation, rhFN1.2 migrates as an approximately 85-100 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 97 % as determined by SDS-PAGE
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Activity
Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When 5 × 10E4 cells/well areadded to CD4 coated plates (1.25μg/mL and 100μL/well),approximately 50%-80% will adhere specifically after 30 minutes at 37℃.
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Validations
Human Fibronectin / Fibronectin Fragment 2 Protein (His Tag) SDS-PAGE
Related Products / Services
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