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Product Name
FGFBP3 antibody
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Description
FGFBP3 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A431 cells, A549 cells, HEK-293 cells, human testis tissue, mouse brain tissue, SH-SY5Y cells. Positive FC detected in HeLa cells. Positive IHC detected in human colon tissue. Positive IF detected in A431 cells. Observed molecular weight by Western-blot: 60-65 kda
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
C10orf13 antibody; FGF binding protein 3 antibody; FGF BP3 antibody; FGFBP 3 antibody; FGFBP3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FGFBP3 recombinant protein (Accession Number: NM_152429). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:110639(FGFBP3 Antibody) at dilution of 1:300
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110639(FGFBP3 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:110639(FGFBP3 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:110639(FGFBP3 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of A431 cells using Catalog No:110639(FGFBP3 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HeLa cells were stained with 0.2ug FGFBP3 antibody (Catalog No:110639, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
FGFBP3 also named as C10orf13 or FGF binding protein 3 is 258 amino acid protein, which belongs to the fibroblast growth factor binding family. FGFBP3 is a secreted protein and may play a role in the regulation of gene transcription. The molecular weight of FGFBP3 is 29kDa, but our results detected the 60-65kDa protein by western blot. We infer that FGFBP3 may exist as dimer.
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