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Product Name
FGF5 antibody
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Description
FGF5 Rabbit Polyclonal antibody. Positive IP detected in A375 cells. Positive WB detected in A375 cells, NIH/3T3 cells, SH-SY5Y cells. Positive IHC detected in human stomach tissue, human placenta tissue. Positive IF detected in NIH/3T3 cells. Observed molecular weight by Western-blot: 34 kDa
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Tested applications
ELISA, WB, IF, IP, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
FGF 5 antibody; FGF5 antibody; fibroblast growth factor 5 antibody; HBGF 5 antibody; Smag 82 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FGF5 recombinant protein (Accession Number: NM_004464). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:50-1:500
IF: 1:20-1:200
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Validations
A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:110635(FGF5 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human stomach tissue slide using Catalog No:110635(FGF5 Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human stomach tissue slide using Catalog No:110635(FGF5 Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of NIH/3T3 cells using Catalog No:110635(FGF5 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IP Result of anti-FGF5 (IP:Catalog No:110635, 4ug; Detection:Catalog No:110635 1:500) with A375 cells lysate 3200ug.
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Background
FGF5 represents fibroblast growth factor 5
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References
- Wang T, Zhang Y, Wang HD. Alpaca fiber growth is mediated by microRNA let-7b via down-regulation of target gene FGF5. Genetics and molecular research : GMR. 14(4):13754-63. 2015.
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