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Product Name
FGD1 Polyclonal Antibody
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Description
Polyclonal antibody to FGD1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
FGD1 antibody; AAS antibody; FGDY antibody; MRXS16 antibody; ZFYVE3 antibody; FYVE, RhoGEF and PH domain containing 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 700-800 of human FGD1 (NP_004454.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - FGD1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using FGD1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Immunohistochemistry - FGD1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using FGD1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - FGD1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using FGD1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - FGD1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using FGD1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - FGD1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human placenta using FGD1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - FGD1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using FGD1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - FGD1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using FGD1 antibody at dilution of 1:100 (40x lens).
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Background
Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"