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Product Name
FEN1 antibody
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Description
FEN1 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IF detected in NIH/3T3 cells. Positive IP detected in NIH/3T3 cells. Positive WB detected in NIH/3T3 cells, HeLa cells, U-937 cells. Observed molecular weight by Western-blot: 48 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DNase IV antibody; FEN 1 antibody; FEN1 antibody; Flap endonuclease 1 antibody; flap structure endonuclease 1 antibody; hFEN 1 antibody; Maturation factor 1 antibody; MF1 antibody; RAD2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FEN1 recombinant protein (Accession Number: NM_004111). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:110618(FEN1 antibody) at dilution of 1:500
IP Result of anti-FEN1 (IP:Catalog No:110618, 4ug; Detection:Catalog No:110618 1:500) with NIH/3T3 cells lysate 1200ug.
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:110618(FEN1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using Catalog No:110618(FEN1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
FEN1 represents flap structure-specific endonuclease 1
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