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Product Name
FDX1 antibody
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Description
FDX1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human kidney tissue, human liver tissue. Positive WB detected in HepG2 cells, A549 cells, HEK-293 cells, PC-3 cells. Observed molecular weight by Western-blot: 14 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Adrenal ferredoxin antibody; Adrenodoxin antibody; mitOChondrial antibody; ADX antibody; FDX antibody; FDX1 antibody; ferredoxin 1 antibody; Hepatoredoxin antibody; LOH11CR1D antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FDX1 recombinant protein (Accession Number: NM_004109). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:110609(FDX1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human kidney using Catalog No:110609(FDX1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:110609(FDX1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:110609(FDX1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
FDX1(Ferredoxin-1) is also named as ADX, FDX, YAH1, LOH11CR1D and belongs to the adrenodoxin/putidaredoxin family. It is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450 and it can can only reduce mitochondrial CYP enzymes that are essential in adrenal steroidogenesis, bile acid formation, and vitamin D synthesis. The full length has a transit peptide of 60 amino acid.
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