-
Product Name
FDFT1 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to FDFT1
-
Tested applications
WB, IHC, IF
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
FDFT1 antibody; DGPT antibody; ERG9 antibody; SQS antibody; SS antibody; squalene synthase antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human FDFT1 (NP_004453.3).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - FDFT1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using FDFT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 60s.
Immunohistochemistry - FDFT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse testis using FDFT1 antibody at dilution of 1:100 (20x lens).
Immunohistochemistry - FDFT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using FDFT1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - FDFT1 Polyclonal Antibody
Immunofluorescence analysis of NIH-3T3 cells using FDFT1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"