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Product Name
FBXW4 Polyclonal Antibody
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Description
Polyclonal antibody to FBXW4
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
FBXW4 antibody; DAC antibody; FBW4 antibody; FBWD4 antibody; SHFM3 antibody; SHSF3 antibody; F-box/WD repeat-containing protein 4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 143-412 of human FBXW4 (NP_071322.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - FBXW4 Polyclonal Antibody
Western blot analysis of extracts of mouse brain, using FBXW4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Background
Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. Likely to be involved in key signaling pathways crucial for normal limb development. May participate in Wnt signaling.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"