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Product Name
FBXO7 Polyclonal Antibody
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Description
Polyclonal antibody to FBXO7
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
FBXO7 antibody; FBX antibody; FBX07 antibody; FBX7 antibody; PARK15 antibody; PKPS antibody; F-box protein 7 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 353-522 of human FBXO7 (NP_036311.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - FBXO7 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using FBXO7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - FBXO7 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using FBXO7 antibody .
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Background
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes BIRC2 and DLGAP5. Plays a role downstream of PINK1 in the clearance of damaged mitochondria via selective autophagy (mitophagy) by targeting PRKN to dysfunctional depolarized mitochondria. Promotes MFN1 ubiquitination.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"