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Product Name
FBXO7 antibody
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Description
FBXO7 Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue. Positive IP detected in mouse testis tissue. Positive WB detected in HeLa cells, BxPC-3 cells, human brain tissue, human placenta tissue, K-562 cells, MCF7 cells, mouse testis tissue. Observed molecular weight by Western-blot: 58.5 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
DKFZp686B08113 antibody; F box only protein 7 antibody; F box protein 7 antibody; FBX antibody; FBX07 antibody; FBX7 antibody; FBXO7 antibody; PARK15 antibody; PKPS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FBXO7 recombinant protein (Accession Number: NM_012179). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110558(FBXO7 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:110558(FBXO7 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-FBXO7 (IP:Catalog No:110558, 3ug; Detection:Catalog No:110558 1:800) with mouse testis tissue lysate 4000ug.
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Background
FBXO7, also named as FBX7, is a substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. FBXO7 recognizes BIRC2 and DLGAP5. It function in phosphorylation-dependent ubiquitination.
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References
- Chen MC, Chen CH, Chuang HC, Kulp SK, Teng CM, Chen CS. Novel mechanism by which histone deacetylase inhibitors facilitate topoisomerase IIα degradation in hepatocellular carcinoma cells. Hepatology (Baltimore, Md.). 53(1):148-59. 2011.
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