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Product Name
FBXO43 antibody
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Description
FBXO43 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells. Positive IF detected in HEK-293 cells. Observed molecular weight by Western-blot: ~70 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
EMI2 antibody; Endogenous meiotic inhibitor 2 antibody; ERP1 antibody; F box only protein 43 antibody; F box protein 43 antibody; Fbp5B antibody; Fbx43 antibody; FBXO43 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001029860). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:110555(FBXO43 Antibody) at dilution of 1:300
Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using Catalog No:110555(FBXO43 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
FBXO43, also named as EMI2 and FBP5B, is required to establish and maintain the arrest of oocytes at the second meiotic metaphase until fertilization. It acts by inhibiting the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. FBXO43 recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. We got ~70 kDa band in our detection.
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