FBXO31 Polyclonal Antibody
Polyclonal antibody to FBXO31
FBXO31 antibody; FBX14 antibody; FBXO14 antibody; Fbx31 antibody; MRT45 antibody; pp2386 antibody; F-box protein 31 antibody
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 240-539 of human FBXO31 (NP_079011.3).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
Western blot - FBXO31 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using FBXO31 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in G1 arrest following DNA damage. Specifically recognizes phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, resulting in G1 arrest. May act as a tumor suppressor.
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