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Product Name
FBXO22 antibody
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Description
FBXO22 Rabbit Polyclonal antibody. Positive IHC detected in human tonsil tissue, human liver cancer tissue. Positive WB detected in HeLa cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 45 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
F box only protein 22 antibody; F box protein 22 antibody; F box protein FBX22p44 antibody; FBX22 antibody; FBXO22 antibody; FISTC1 antibody; FLJ13986 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FBXO22 recombinant protein (Accession Number: XM_011521447). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110550(FBXO22 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human tonsil using Catalog No:110550(FBXO22 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human tonsil using Catalog No:110550(FBXO22 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-FBXO22 (IP:Catalog No:110550, 4ug; Detection:Catalog No:110550 1:500) with HeLa cells lysate 4000ug.
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Background
FBXO22, also named as FBX22, is a substrate-recognition of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. This antibody recognizes isoform 1 (45 kDa) and isoform 3 (30-33 kDa).
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References
- Hwang CY, Kim K, Choi JY. Quantitative proteome analysis of age-related changes in mouse gastrocnemius muscle using mTRAQ. Proteomics. 14(1):121-32. 2014.
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