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Product Name
FBXL5 Polyclonal Antibody
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Description
Polyclonal antibody to FBXL5
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
FBXL5 antibody; FBL4 antibody; FBL5 antibody; FLR1 antibody; F-box/LRR-repeat protein 5 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human FBXL5 (NP_036293.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:100 -
Validations
Western blot - FBXL5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using FBXL5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry - FBXL5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using FBXL5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - FBXL5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon carcinoma using FBXL5 antibody at dilution of 1:100 (40x lens).
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Background
Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"