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Product Name
FBX15 antibody
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Description
FBX15 Rabbit Polyclonal antibody. Positive WB detected in A2780 cells, mouse ovary tissue. Observed molecular weight by Western-blot: 55kd
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Tested applications
ELISA, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
F box only protein 15 antibody; F box protein 15 antibody; FBX15 antibody; FBXO15 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FBX15 recombinant protein (Accession Number: XM_017025604). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
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Validations
A2780 cells were subjected to SDS PAGE followed by western blot with Catalog No:110540(FBXO15 antibody) at dilution of 1:1500
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Background
F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins, and they are a family of eukaryotic proteins characterized by an approximately 40 amino acid motif. SCF complex, a class of ubiquitin ligases, consists of invariable components, Skp1 and Cullin, and variable components of F-box proteins, which have a primary role in determining substrate specificity. The F-box proteins are divided into 3 classes: FBWs containing WD-40 domains, FBLs containing leucine-rich repeats, and FBXs containing either different protein-protein interaction modules or no recognizable motifs. FBOX15 (or FBX15) belongs to the FBXs class, and 2 isoforms produced by alternative splicing has been described. This antibody is expected detect both isoforms.
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