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Product Name
FASTK Polyclonal Antibody
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Description
Polyclonal antibody to FASTK
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
FASTK antibody; FAST antibody; fas-activated serine/threonine kinase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 360-549 of human FASTK (NP_006703.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100 -
Validations
Western blot - FASTK Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using FASTK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - FASTK Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat liver using FASTK antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - FASTK Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human kidney using FASTK antibody at dilution of 1:100 (40x lens).
Immunofluorescence - FASTK Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using FASTK antibody . Blue: DAPI for nuclear staining.
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Background
Phosphorylates the splicing regulator TIA1, thereby promoting the inclusion of FAS exon 6, which leads to an mRNA encoding a pro-apoptotic form of the receptor.; Isoform 4: Required for the biogenesis of some mitochondrial-encoded mRNAs, specifically stabilizes ND6 (NADH dehydrogenase complex subunit 6) mRNA, and regulates its levels.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"