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Product Name
FAM65B antibody
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Description
FAM65B Rabbit Polyclonal antibody. Positive WB detected in human skeletal muscle tissue. Observed molecular weight by Western-blot: 115kd,66kd
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Tested applications
ELISA, WB
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
FAM65B antibody; C6orf32 antibody; KIAA0386 antibody; Protein FAM65B antibody; DIFF40 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FAM65B recombinant protein (Accession Number: XM_017011527). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
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Validations
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:110596(FAM65B antibody) at dilution of 1:500
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Background
FAM65B is also named as C6orf32, DIFF48, KIAA0386, PL48 and belongs to the FAM65 family.It has 2 isoforms produced by alternative splicing.Isoform 2 play a role in promoting myogenic cell differentiation, cytoskeletal rearrangement and filopodia formation(PMID:17150207). FAM65B is expressed in mononuclear cells, not in myotubes, upon induction of differentiation and cellular fusion, and expression is upregulated during human myoblast differentiation at both the mRNA and protein levels(PMID:17150207).This antibody is specific to FAM65B.
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References
- Gao K, Tang W, Li Y. Front-signal-dependent accumulation of the RHOA inhibitor FAM65B at leading edges polarizes neutrophils. Journal of cell science. 128(5):992-1000. 2015.
- Balasubramanian A, Kawahara G, Gupta VA. Fam65b is important for formation of the HDAC6-dysferlin protein complex during myogenic cell differentiation. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 28(7):2955-69. 2014.
- Diaz-Horta O, Subasioglu-Uzak A, Grati M. FAM65B is a membrane-associated protein of hair cell stereocilia required for hearing. Proceedings of the National Academy of Sciences of the United States of America. 111(27):9864-8. 2014.
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