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Product Name
FAM49B antibody
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Description
FAM49B Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human kidney tissue, human brain tissue, human pancreas tissue. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, human placenta tissue, human testis tissue. Observed molecular weight by Western-blot: 37 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BM 009 antibody; DKFZp686B04128 antibody; FAM49B antibody; Protein FAM49B antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FAM49B recombinant protein (Accession Number: NM_001256763). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110592(FAM49B antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:110592(FAM49B antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:110592(FAM49B antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using FAM49B antibody Catalog No:110592 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-FAM49B (IP:Catalog No:110592, 4ug; Detection:Catalog No:110592 1:500) with mouse brain tissue lysate 4000ug.
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Background
FAM49B represents family with sequence similarity 49, member B
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