-
Product Name
FAM124B antibody
- Documents
-
Description
FAM124B Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human lung cancer tissue, human lung tissue, mouse heart tissue. Positive WB detected in K-562 cells, HeLa cells, HepG2 cells, MCF7 cells, mouse heart tissue, mouse testis tissue. Observed molecular weight by Western-blot: 51kd
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
FAM124B antibody; FLJ22746 antibody; Protein FAM124B antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of FAM124B recombinant protein (Accession Number: XM_047445888). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:110467(FAM124B antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:110467(FAM124B antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:110467(FAM124B antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.
Immunofluorescent analysis of Hela cells, using FAM124B antibody Catalog No:110467 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
-
Background
FAM124B is identified as a novel component of CHD7 and CHD8 containing complex. It is a mainly nuclear localized protein with a widespread expressed. Fam124B is a novel protein possibly involved in the pathogenesis of CHARGE syndrome and neurodevelopmental disorders.
-
References
- Batsukh T, Schulz Y, Wolf S. Identification and characterization of FAM124B as a novel component of a CHD7 and CHD8 containing complex. PloS one. 7(12):e52640. 2012.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"