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FAM117B antibody

Cat.#: 110462

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Product Information

  • Product Name
    FAM117B antibody
  • Documents
  • Description
    FAM117B Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells. Positive IP detected in HEK-293 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 66 kDa
  • Tested applications
    ELISA, IHC, IF, WB, IP
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    ALS2CR13 antibody; DKFZp686H01244 antibody; FAM117B antibody; FLJ38771 antibody; Protein FAM117B antibody
  • Immunogen
    Human FAM117B Recombinant protein (GST tag)
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of FAM117B recombinant protein (Accession Number: BC106906). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110462(FAM117B antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110462(FAM117B antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:110462(FAM117B antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:110462(FAM117B antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of HepG2 cells, using FAM117B antibody Catalog No:110462 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells, using FAM117B antibody Catalog No:110462 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    IP Result of anti-FAM117B (IP:Catalog No:110462, 4ug; Detection:Catalog No:110462 1:500) with HEK-293 cells lysate 920ug.

    IP Result of anti-FAM117B (IP:Catalog No:110462, 4ug; Detection:Catalog No:110462 1:500) with HEK-293 cells lysate 920ug.

  • Background
    FAM117B, also named as ALS2CR13, is mapped in the genomic region covering the complete candidate region for Amyotrophic lateral sclerosis 2 (ALS2). This antibody is specific to FAM117B.
  • References
    • Hast BE, Goldfarb D, Mulvaney KM. Proteomic analysis of ubiquitin ligase KEAP1 reveals associated proteins that inhibit NRF2 ubiquitination. Cancer research. 73(7):2199-210. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"