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Product Name
FAF1 Polyclonal Antibody
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Description
Polyclonal antibody to FAF1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
FAF1 antibody; CGI-03 antibody; HFAF1s antibody; UBXD12 antibody; UBXN3A antibody; hFAF1 antibody; FAS-associated factor 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human FAF1
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - FAF1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using FAF1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Immunohistochemistry - FAF1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using FAF1 antibody (40x lens).
Immunohistochemistry - FAF1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using FAF1 antibody (40x lens).
Immunohistochemistry - FAF1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using FAF1 antibody (40x lens).
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Background
Ubiquitin-binding protein. Required for the progression of DNA replication forks by targeting DNA replication licensing factor CDT1 for degradation. Potentiates but cannot initiate FAS-induced apoptosis (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"