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Product Name
FAF1 antibody
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Description
FAF1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, K-562 cells, mouse brain tissue, mouse testis tissue. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 66-74kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CGI 03 antibody; FAF1 antibody; FAS assOCiated factor 1 antibody; FLJ37524 antibody; hFAF1 antibody; HFAF1s antibody; UBXD12 antibody; UBXN3A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FAF1 recombinant protein (Accession Number: NM_007051). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110451(FAF1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:110451(FAF1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:110451(FAF1 antibody) at dilution of 1:50 (under 40x lens)
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Background
FAF1 was detected most abundant in testis, slightly less abundant in skeletal muscle and heart, followed by prostate, thymus, ovary, small intestine, and colon, but not in the peripheral blood leukocytes.The N-terminal region (amino acid 1∼201) including the upstream ubiquitin homology domain of hFAF1 could bind with the death domain of Fas, which mediates programmed cell death, also called apoptosis, in a number of organ systems, notably the immune and nervous systems.
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References
- Cheng K, Bai L, Belluscio L. Fas-associated factor 1 as a regulator of olfactory axon guidance. The Journal of neuroscience : the official journal of the Society for Neuroscience. 31(33):11905-13. 2011.
- Sul JW, Park MY, Shin J. Accumulation of the parkin substrate, FAF1, plays a key role in the dopaminergic neurodegeneration. Human molecular genetics. 22(8):1558-73. 2013.
- Kim JM, Kim SS, Lee YD. Fas-associated factor 1 promotes in neurofibrillary tangle-mediated cell death of basal forebrain cholinergic neurons in P301L transgenic mice. Neuroreport. 26(13):767-72. 2015.
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