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Product Name
Factor XII antibody
- Documents
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Description
Factor XII Mouse Monoclonal antibody. Positive IHC detected in human liver tissue. Positive IF detected in HepG2 cells. Positive WB detected in K562 cells. Observed molecular weight by Western-blot: 28 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human,Mouse; other species not tested.
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Alternative names
Factor XII antibody; Coagulation factor XII antibody; F12 antibody; HAE3 antibody; HAEX antibody; HAF antibody; Hageman factor antibody
- Immunogen
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of Factor XII recombinant protein (Accession Number: BC012390). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:107199(F12 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:107199(F12 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:107199(F12 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using F12 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
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Background
Factor XII (F XII, Hageman factor) is a 80kD, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. When plasma comes into contact with negatively charged surfaces such as glass, kaolin, or dextran sulfate, F XII is bound to the surface and undergoes limited proteolysis by plasma kallikrein, resulting in two active enzyme forms, ɑ-F XIIa and β-F XIIa. ɑ-F XIIa consisting of a 28kD light chain containing the protease domain, and a 52kD heavy chain containing the anionic surface-binding domain. β-F XIIa consists of two disulfide-linked chains, a 2 kD light chain and a 28 kD heavy chain containing the catalytic domain. Defects in Factor XII do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged.
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