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Product Name
EZH2 antibody
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Description
EZH2 Rabbit Polyclonal antibody. Positive IP detected in HepG2 cells. Positive WB detected in Raji cells, A431 cells, BxPC-3 cells, DU 145 cells, HEK-293 cells, HepG2 cells, Jurkat cells, mouse kidney tissue, NIH/3T3 cells, rat kidney tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 90-102 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
Enhancer of zeste homolog 2 antibody; ENX 1 antibody; EZH1 antibody; EZH2 antibody; KMT6 antibody; Lysine N methyltransferase 6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EZH2 recombinant protein (Accession Number: NM_004456). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IF: 1:20-1:200
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Validations
Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:110425(EZH2 antibody) at dilution of 1:1500
IP Result of anti-EZH2 (IP:Catalog No:110425, 4ug; Detection:Catalog No:110425 1:1500) with HepG2 cells lysate 4000ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:110425(EZH2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)
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References
- Liang J, Zhang Y, Jiang G. MiR-138 induces renal carcinoma cell senescence by targeting EZH2 and is downregulated in human clear cell renal cell carcinoma. Oncology research. 21(2):83-91. 2013.
- Hou Z, Zhao W, Zhou J. A long noncoding RNA Sox2ot regulates lung cancer cell proliferation and is a prognostic indicator of poor survival. The international journal of biochemistry & cell biology. 53:380-8. 2014.
- Cai D, Yuan M, Jia Y, Liu H, Hu Y, Zhao R. Maternal gestational betaine supplementation-mediated suppression of hepatic cyclin D2 and presenilin1 gene in newborn piglets is associated with epigenetic regulation of the STAT3-dependent pathway. The Journal of nutritional biochemistry. 26(12):1622-31. 2015.
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