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Product Name
EYA3 Polyclonal Antibody
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Description
Polyclonal antibody to EYA3
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
EYA3 antibody; eyes absent homolog 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human EYA3 (NP_001981.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - EYA3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using EYA3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - EYA3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using EYA3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - EYA3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human kidney using EYA3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - EYA3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric using EYA3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - EYA3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse liver using EYA3 antibody at dilution of 1:100 (40x lens).
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Background
Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1, and seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity (By similarity). May be involved in development of the eye.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"