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Product Name
EXTL3 Polyclonal Antibody
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Description
Polyclonal antibody to EXTL3
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
EXTL3 antibody; BOTV antibody; EXTL1L antibody; EXTR1 antibody; REGR antibody; RPR antibody; ISDNA antibody; exostosin-like 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 52-220 of human EXTL3 (NP_001431.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
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Validations
Western blot - EXTL3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using EXTL3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
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Background
Glycosyltransferase which regulates the biosynthesis of heparan sulfate (HS). Important for both skeletal development and hematopoiesis, through the formation of HS proteoglycans (HSPGs). Required for the function of REG3A in regulating keratinocyte proliferation and differentiation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"