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Product Name
EXT1 Polyclonal Antibody
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Description
Polyclonal antibody to EXT1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
EXT1 antibody; EXT antibody; LGCR antibody; LGS antibody; TRPS2 antibody; TTV antibody; exostosin-1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 467-746 of human EXT1 (NP_000118.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:10 - 1:100 -
Validations
Western blot - EXT1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using EXT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - EXT1 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using EXT1 antibody . Blue: DAPI for nuclear staining.
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Background
Glycosyltransferase required for the biosynthesis of heparan-sulfate. The EXT1/EXT2 complex possesses substantially higher glycosyltransferase activity than EXT1 or EXT2 alone. Appears to be a tumor suppressor. Required for the exosomal release of SDCBP, CD63 and syndecan.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"