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Product Name
EXOSC3 antibody
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Description
EXOSC3 Rabbit Polyclonal antibody. Positive IP detected in A2780 cells. Positive WB detected in PC-3 cells, A2780 cells. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 31 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
bA3J10.7 antibody; CGI 102 antibody; EXOSC3 antibody; exosome component 3 antibody; hRrp 40 antibody; hRrp40p antibody; p10 antibody; RP11 3J10.8 antibody; RRP40 antibody; Rrp40p antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EXOSC3 recombinant protein (Accession Number: NM_016042). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:110410(EXOSC3 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:110410(EXOSC3 antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:110410(EXOSC3 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-EXOSC3 (IP:Catalog No:110410, 4ug; Detection:Catalog No:110410 1:500) with A2780 cells lysate 800ug.
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Background
RNA exosomes are multi-subunit complexes conserved throughout evolution, and they are emerging as the major cellular machinery for processing, surveillance and turnover of a diverse spectrum of coding and noncoding RNA substrates essential for viability[PMID:22544365]. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts [PMID:11782436]. EXOSC3 is a non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and involves in a multitude of cellular RNA processing and degradation events. EXOSC3 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of Rnase PH-domain subunits through contacts with EXOSC9 and EXOSC5 [PMID:21255825].
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References
- Lloret-Llinares M, Mapendano CK, Martlev LH, Lykke-Andersen S, Jensen TH. Relationships between PROMPT and gene expression. RNA biology. 13(1):6-14. 2016.
- Boczonadi V, Müller JS, Pyle A. EXOSC8 mutations alter mRNA metabolism and cause hypomyelination with spinal muscular atrophy and cerebellar hypoplasia. Nature communications. 5:4287. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"