-
Product Name
EXOSC10 antibody
- Documents
-
Description
EXOSC10 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive IF detected in NIH/3T3 cells.
-
Tested applications
ELISA, IHC, IF
-
Species reactivity
Human; other species not tested.
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of EXOSC10 recombinant protein (Accession Number: NM_001001998). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:110508(EXOSC10 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of NIH/3T3 cells using Catalog No:110508(EXOSC10 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear/nucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.
-
References
- Tseng CK, Wang HF, Burns AM, Schroeder MR, Gaspari M, Baumann P. Human Telomerase RNA Processing and Quality Control. Cell reports. 13(10):2232-43. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"