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Product Name
EXO1 Polyclonal Antibody
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Description
Polyclonal antibody to EXO1
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Tested applications
WB, IF
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Species reactivity
Human
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Alternative names
EXO1 antibody; HEX1 antibody; hExoI antibody; exonuclease 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human EXO1
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IF 1:20 - 1:50 -
Validations
Western blot - EXO1 Polyclonal Antibody
Western blot analysis of extracts of NCI-H460 cells, using EXO1 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
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Background
5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"