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Product Name
EXO1 antibody
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Description
EXO1 Rabbit Polyclonal antibody. Positive IP detected in mouse thymus tissue. Positive WB detected in Jurkat cells. Observed molecular weight by Western-blot: 115-120 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human; other species not tested.
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Alternative names
EXO1 antibody; EXOI antibody; exonuclease 1 antibody; Exonuclease I antibody; HEX1 antibody; hExo1 antibody; hExoI antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EXO1 recombinant protein (Accession Number: XM_011544322). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:110499(EXO1 antibody) at dilution of 1:800
IP Result of anti-EXO1 (IP:Catalog No:110499, 4ug; Detection:Catalog No:110499 1:500) with mouse thymus tissue lysate 2800ug.
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Background
EXO1, also named as HEX1, is a 846 amino acid protein, which belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. EXO1 is highly expressed in bone marrow, testis and thymus and at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach. EXO1 as 5'->3' double-stranded DNA exonuclease, which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. EXO1 functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch.
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References
- Chen X, Paudyal SC, Chin RI, You Z. PCNA promotes processive DNA end resection by Exo1. Nucleic acids research. 41(20):9325-38. 2013.
- Tammaro M, Liao S, Beeharry N, Yan H. DNA double-strand breaks with 5' adducts are efficiently channeled to the DNA2-mediated resection pathway. Nucleic acids research. 44(1):221-31. 2016.
- Wu Y, Lee SH, Williamson EA. EEPD1 Rescues Stressed Replication Forks and Maintains Genome Stability by Promoting End Resection and Homologous Recombination Repair. PLoS genetics. 11(12):e1005675. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"